Lycor dot blot protocol12/30/2023 ![]() Digital imaging is useful because it captures more data than x-ray film in a single exposure and reduces the need for multiple exposures. LI-COR can help bypass any potential x-ray film constraints by facilitating a transition to digital imaging. It is therefore important to understand the variability of ECL in its entirety before proceeding with experimentation. ![]() Overall, these factors make it difficult to achieve precise quantification. Additionally, chemiluminescence is unable to multiplex signals or simultaneously detect two targets with the same molecular weight in a single sample that is on the same blot. This makes it challenging to accurately quantify the protein abundance on the membrane. Ultimately, it can be difficult to capture the enzymatic reaction for each target simultaneously and at the same point each time.
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